Student Research Projects from Fall 2017
In Fall 2017, student research teams were challenged to propose and pursue a single focused research project for the semester. All students studied cystic fibrosis and designed PCR-based diagostic assays for mutations known to cause that disease. Each group wrote manuscripts gave numerous talks. The reseach assignment involved an extra "control" experiment, where each group was required to also find an existing published PCR assay for the same gene and replicate the experiment using the published primer sequences. These are often referred to as the "control" primers in the final reports/manuscripts.

Student websites reporting their findings at the end of the independent investigations from Fall Semester 2017

R117H: Detection of the R117H Mutation in the Human CFTR Genome for Mild Cystic Fibrosis Utilizing Allele Specific PCR

G542X: Inconclusive Diagnostic Assay to Identify the G542X Mutation of the CFTR Gene in Human Epithelial Cells Using ASPCR.

N1303K: Successfully identifying the genotype of N1303K CFTR gene mutation using ASPCR on human buccal cells

233dupT: Diagnosis of Heterozygous CF Patients afflicted with c.233dupT Mutation using Allele Specific PCR

W1282X-2: Genotypic Identification of the W1282X CFTR Mutation in Cystic Fibrosis Using Allele Specific PCR with Human Buccal Cells
W1282X: PCR test built to amplify exon 20 of CFTR gene in purified cheek cells with an Rz gene control test

3849: In search of locus of additional HphI recognition site in a CF novel mutation using H. sapien DNA, PCR, and HphI analysis.

5T mutation: Identifying the 5T mutation of the CFTR gene using buccal cells from human test subjects and allele specific PCR